Albumin, Egg White

AFpak ACA-894 (an affinity column) has as the ligand Concanavalin A (a lectin type). This column is recommended for the analysis of polysaccharides and glycoproteins.

Sample : Ovalbumin

Column       : Shodex AFpak ACA-894 (8.0mmID*50mm)
Eluent       : (A); 0.02M Tris-HCl(pH7.4) + 0.5M NaCl
               (B); (A) + 0.05M alpha-Methyl-D(+)-glucoside
               Linear gradient: (A) to (B) for 60min
Flow rate    : 0.3mL/min
Detector     : UV(280nm)
Column temp. : Room temp.