Analysis of Metabolites from Nitrogen Containing Compounds

Urea, creatine, creatinine were separated using ion (cation) chromatography column, IC YS-50. Urea and creatinine are known as final metabolites of nitrogen containing compounds.

Sample : 10micro-L
1. Urea 0.06%
2. Creatine 0.001%
3. Creatinine 0.001%

Column       : Shodex IC YS-50 (4.6mmID*125mm)
Eluent       : 10mM H3PO4 aq.
Flow rate    : 1.0mL/min
Detector     : UV(210nm)
Column temp. : 40deg-C