Analysis of Oligopeptides with Acidic and Alkaline Eluent

Oligopeptides were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography) with acidic and alkaline eluent.
Please refer to Effect of Eluent pH (ODP).

Sample :
1. Glu-Gly-Phe
2. Met-Enkephalin
3. Leu-Enkephalin
4. Leu-Trp-Met

Column       : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent       : (1); 0.05% TFA aq./CH3CN=85/15
               (2); 50mM Ammonium acetate(pH10.0)/CH3CN=85/15
Flow rate    : 1.0mL/min
Detector     : UV(220nm)
Column temp. : 30deg-C