Angiotensins were analyzed using IEC SP-825 (a column for strong cation exchange chromatography). Angiotensins are physically active peptides and cause blood pressure to rise. Angiotensin I is a decapeptide, Angiotensin II an octapeptide and Angiotensis III a hexapeptide. These three components separated very well by gradient elution with increasing salt concentration.
Sample : 250micro-L
1. Angiotensin II
2. Angiotensin I
3. Angiotensin III
Column : Shodex IEC SP-825 (8.0mmID*75mm)
Eluent : (A); 20mM Sodium phosphate buffer(pH7.0)
(B); (A) + 0.5M NaCl
Linear gradient: 0min to 60min, (A) to (B)
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : Room temp.