beta Lactoglobulin Subfractions

beta Lactoglobulin subfractions were analyzed using Asahipak ES-502N 7C (a column for weak anion exchange chromatography).

Sample : beta-Lactoglobulin

Column       : Shodex Asahipak ES-502N 7C (7.5mmID*100mm)
Eluent       : (A); 20mM Piperazine(pH5.0)
               (B); (A) + 300mM NaCl
               Linear gradient: 0min to 12min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : 30deg-C