Biotin 4-Aminobenzoic Acid

AFpak AAV-894 an affinity column has avidin (a protein in egg white) as a ligand. This column is recommended for the analysis of biotin derivatives.

Sample : Biotin 4-aminobenzoic acid

Column       : Shodex AFpak AAV-894 (8.0mmID*50mm)
Eluent       : (A); 0.01M Sodium acetate buffer(pH7.0) + 0.15M NaCl
               (B); 0.1M Acetic acid/Isopropanol=50/50
               Step gradient: (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : Room temp.