Calibration Curves of KW400-4F (2) (Proteins)

KW400-4F series is aqueous SEC (GFC) columns and downsized KW-800 series. Figure is calibration curves of KW400-4F series columns with pullulan. KW405-4F newly line-upped column, and it was designed having larger pore size than KW-804. KW405-4F can analyze high molecular weight substances more than 1,000,000 as protein like agglomerated protein. KW400-4F series can be applied wider range of molecular weight substances than KW-800 series.

Sample : Proteins

Columns      : Shodex KW400-4F series (4.6mmID*300mm each)
Eluent       : 50mM Sodium phosphate buffer (pH7.0) + 0.3M NaCl
Flow rate    : 0.33mL/min
Detector     : UV(280nm)
Column temp. : 25deg-C