Chiral Separation Columns

To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be caluculated by the formula in the right figure.
For the columns whose test conditions are shown with blue letters, the column performance should be measured by resolution, and not plate number.

Column type
Test conditions
(1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector
CDA-453 HQ (1)0.01% 6-Fluoro-tryptophan (2)10micro-L (3)H2O (4)0.3mL/min (5)20deg-C (6)UV(254nm)
CDB-453 HQ (1)0.01% DL-Met β-naphthylamide (2)10micro-L (3)1.7% TEA/Acetic acid(pH4.0) + 0.1M NaCl in 20% CH3CN + 80% H2O (4)0.2mL/min (5)20deg-C (6)UV(254nm)
CDC-453 HQ (1)0.02% Dancyl-leucine (2)20micro-L (3)1% Acetic acid + 0.2M NaCl in 10% CH3CN + 90% H2O (4)0.3mL/min (5)Ambient (6)UV(254nm)
CDBS-453 (1)0.1% Nefopam-HCl (2)10micro-L (3)CH3CN/(1% Acetic acid + 0.2M NaCl) aq.=30/70 (4)0.5mL/min (5)Ambient (6)UV(254nm)
CRX-853 (1)0.1% RS-Mandelic acid (2)20micro-L (3)0.25mM CuSO4 aq. (4)1.0mL/min (5)Ambient (6)UV(280nm)
ABA-894 (1)0.1% DL-Tryptophan (2)3micro-L (3)50mM Tris-HCl buffer + 0.15M(pH7.4) (4)1.0mL/min (5)Ambient (6)UV(280nm)