Collagen and BSA

Collagen and BSA were analyzed using Asahipak ES-502N 7C (a column for weak anion exchange chromatography).

Sample :
1. Collagen
Pepsin-digested product of collagen
soluble in acid extracted from pig skin
2. BSA

Column       : Shodex Asahipak ES-502N 7C (7.5mmID*100mm)
Eluent       : 20mM Monoethanolamine buffer(pH9.5) + 500mM NaCl 
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : 30deg-C

Courtesy of Dr. Shirai, Tokyo University of Agriculture and Technology