Comparison of Silica-based Column with Polymer-based Column

Asahipak ODP columns are suitable for the analysis of macromolecules such as proteins. A comparison of resolution between the polymer-based (ODP) and the silica-based (ODS) columns shows that the resolution of the former is better.

Sample :
1. Ribonuclease, 2. Cytochrome c, 3. Lysozyme, 4. Chymotrypsinogen A, 5. Ovalbumin

Columns      : Shodex Asahipak ODP-50 6D(6.0mmID*150mm), ODS(6.0mmID*150mm)
Eluent       : 0.05% TFA aq./CH3CN
               Linear gradient: 0 to 30min, 90/10 to 40/60
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : 30deg-C