Crude Pronase E

Crude pronase E was analyzed using Asahipak GS-320 7G ( a multimode column ).
GS-320 7G is an old type of GS-320 HQ.

Sample : Crude pronase E

Column       : Shodex Asahipak GS-320 7G (7.5mmID*500mm)
Eluent       : 1/15M KH2PO4 + 1/15M Na2HPO4(pH7.4)
Flow rate    : 1.0mL/min
Detector     : UV(210nm)
Column temp. : Room temp.

H. Wada et al., J. Chromatogr. , 320 (1985) 369