Cyclic AMP and AMP

As Asahipak GS-320 HQ is packed with a packing material bonded with trace amounts of ion exchange groups, the multimode, which combines size exclusion mode predominately with ion exchange mode and reversed phase mode, can be used for the separation of nucleotides.

Sample : 20micro-g/mL each, 20micro-L
1. 2′AMP
2. 3′AMP
3. cAMP

Column       : Shodex Asahipak GS-320 HQ (7.5mmID*300mm)
Eluent       : 200mM NaH2PO4 aq./ 200mM Na2HPO4 aq.=500/15
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : 30deg-C