Cytochrome P450 in Rat Liver Microsome

Chytochrome P450 in rat liver microsome was analyzed using Asahipak ES-502C 7C (a column for weak cation exchange chromatography).

Sample : Cytochrome P450 in rat liver microsome

Column       : Shodex Asahipak ES-502C 7C (7.5mmID*100mm)
Eluent       : (A); 20mM Sodium phosphate buffer(pH6.5) + 20% Glycerol + 0.4% Emulgen 911
               (B); (A) + 1.0M Sodium Acetate
               Linear gradient: 0min to 50min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : VIS(417nm)
Column temp. : Room temp.

Courtesy of Dr. Funae, Osaka City University