Dansyl Amino Acids

Dansyl-amino acid was analyzed using ODSpak F-411 (a column for reversed phase chromatography) and a chemiluminescence detectot (CL) and a fluorescence detector detector compared.

Sample :

Column       : Shodex ODSpak F-411 (4.6mmID*150mm)
Eluent       : 0.1M Imidazole Buffer(pH7.0, adjusted by nitrate)/CH3CN=25/75
Flow rate    : 0.5mL/min
Reagent      : [1mM TCPO in Ethyl acetate}/[0.15M H2O2(30%aq) in CH3CN]=25/75
Detector     : CL, Fluorescence detector(Ex. 330nm, Em. 560nm)
Column temp. : 30deg-C