Dipeptides

Dipeptides were analyzed using RSpak DE-613 (a column for reversed phase chromatography).

Sample :
1. Gly-Val
2. Phe-Gly
3. Ala-Phe
4. Val-Phe
5. Tyr-Leu

Column       : Shodex RSpak DE-613 (6.0mmID*150mm)
Eluent       : (A); 0.05% TFA aq./CH3CN=96/4
               (B); 0.05% TFA aq./CH3CN=75/25
               Linear gradient: 0 to 30min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(220nm)
Column temp. : 30deg-C