DNA Fragments Digested by Hae III (DEAE-420N)
DNA fragments digested by Hae III were analyzed using IEC DEAE-420N (a column for weak anion exchange chromatography packed with non-porous gel).
DEAE-420N is an old type of DEAE3N-4T.
Sample : phi-X-174-RF DNA fragment
|
No.
|
base pairs
|
MW (x 105daltons)
|
|
1.
|
1353
|
8.79
|
|
2.
|
1078
|
7.01
|
|
3.
|
872
|
5.67
|
|
4.
|
603
|
3.92
|
|
5.
|
310
|
2.02
|
|
6B.
|
281
|
1.83
|
|
6A.
|
271
|
1.76
|
|
7.
|
234
|
1.52
|
|
8.
|
194
|
1.26
|
|
9.
|
118
|
0.77
|
|
10.
|
72
|
0.47
|
Column : Shodex IEC DEAE-420N (4.6mmID*35mm)
Eluent : (A); 25mM Phosphate buffer(pH5.2)
(B); (A) + 1M NaCl
Linear gradient: 0min to 20min, 75% (B) to 100% (B)
Flow rate : 1.0mL/min
Detector : UV(260nm)
Column temp. : 30deg-C