Effect of Flow Rate in Analysis of Ribosomal RNA

Ribosomal RNA was analyzed using Asahipak GS-620 7G (a column for GFC separation). For general HPLC analysis, a high flow rate of 0.5-1.0mL/min or over is generally used. Whereas, for the GFC analysis of high molecular weight components, it is recommended that the analysis be made at a flow rate as low as possible, e.g. 0.1mL/min to prevent molecular chains from being cut off and to permit a high resolution.
GS-620 7G is an old type of GS-620 HQ.

Sample :
1. Ribosomal RNA-23S
2. Ribosomal RNA-16S
3. Ribosomal RNA-5S

Column       : Shodex Asahipak GS-620 7G (7.5mmID*500mm)
Eluent       : 0.1M Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Detector     : UV(260nm)
Column temp. : 30deg-C