Effect of Injection Volume of Serum

Effect of injection volume when analyzing serum using MSpak GF-310 4B (a column for rapid analysis of drugs) was shown.

Sample : Control serum I Wako
(from Wako Pure Chemical Industries, Ltd.)

Column       : Shodex MSpak GF-310 4B (4.6mmID*50mm)
Eluent       : 50mM CH3COONH4 aq./CH3CN=70/30
Flow rate    : 0.25mL/min
Detector     : UV(220nm)
Column temp. : 30deg-C