When Asahipak NH2P-50 is used, it is recommended to use line filter, NH2P-LF with it. Figure (A) and (B) show the effect of NH2P-LF. Figure (A) shows that the column resolution is same for about 135 hours, however, figure (B) shows that the column resolution decreases just after it is used.
Regarding figures (a), (b) and (c), refer to Effect of Line Filter NH2P-LF (1).
Sample : 0.2% each, 5micro-L
1. meso-Erythritol, 2. Xylitol, 3. Fructose, 4. Mannose, 5. Glucose
6. myo-Inositol, 7. Sucrose, 8. Lactose, 9. Maltose, 10. Raffinose
Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent : CH3CN/H2O=75/25
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 25deg-C