Effect of pH on Elution Pattern (DEAE-420N)
Effect of pH on elution pattern IEC DEAE-420N (a column for weak anion exchange chromatography packed with non-porous gel) is shown.
DEAE-420N is an old type of DEAE3N-4T.
Sample :
1. Conalbumin
2. Transferrin
3. Ovalbumin
4. Trypsin inhibitor
Column : Shodex IEC DEAE-420N (4.6mmID*35mm) Eluent : 25mM Piperazine-HCl buffer(pH6.0) 25mM Piperazine-HCl buffer(pH6.5) 25mM Tris-HCl buffer(pH7.5) 25mM Ethanolamine-HCl buffer(pH9.0) Linear gradient: 0min to 10min, 0M to 0.5M NaCl Flow rate : 1.5mL/min Detector : UV(280nm) Column temp. : Room temp.