Effect of pH on Elution Pattern (DEAE-420N)

Effect of pH on elution pattern IEC DEAE-420N (a column for weak anion exchange chromatography packed with non-porous gel) is shown.
DEAE-420N is an old type of DEAE3N-4T.

Sample :
1. Conalbumin
2. Transferrin
3. Ovalbumin
4. Trypsin inhibitor

Column       : Shodex IEC DEAE-420N (4.6mmID*35mm)
Eluent       : 25mM Piperazine-HCl buffer(pH6.0)
               25mM Piperazine-HCl buffer(pH6.5)
               25mM Tris-HCl buffer(pH7.5)
               25mM Ethanolamine-HCl buffer(pH9.0)
               Linear gradient: 0min to 10min, 0M to 0.5M NaCl
Flow rate    : 1.5mL/min
Detector     : UV(280nm)
Column temp. : Room temp.