Effect of Sample Injection Volume (ODP2 HP)

The relation between sample injection volume and theoretical plate number (TPN) for ODP2 HP 4D (4.6mm ID x 150mm length) is shown. Sample injection volume of 40micro-L and below is recommended for ODP2 HP-4D to achieve maximum efficiency. Sample injection volume of 8micro-L and below is recommended for ODP2 HP-2D (2mm ID x 150mm length).

Sample : 0.6micro-g each
Methyl bezoate
Ratio of TPN [%] : TPN at sample injection of 2µL is set as 100%.

Column       : Shodex ODP2 HP-4D (4.6mmID*150mm)
Eluent       : H2O/CH3CN=55/45
Flow rate    : 1.0mL/min
Detector     : UV(254nm) Phenol, Methyl benzoate
               UV(300nm) Caffeine
Column temp. : 40deg-C