Effect of Sample Injection Volume (ODP2 HP)
The relation between sample injection volume and theoretical plate number (TPN) for ODP2 HP 4D (4.6mm ID x 150mm length) is shown. Sample injection volume of 40micro-L and below is recommended for ODP2 HP-4D to achieve maximum efficiency. Sample injection volume of 8micro-L and below is recommended for ODP2 HP-2D (2mm ID x 150mm length).
Sample : 0.6micro-g each
Phenol
Methyl bezoate
Caffeine
Ratio of TPN [%] : TPN at sample injection of 2µL is set as 100%.
Column : Shodex ODP2 HP-4D (4.6mmID*150mm) Eluent : H2O/CH3CN=55/45 Flow rate : 1.0mL/min Detector : UV(254nm) Phenol, Methyl benzoate UV(300nm) Caffeine Column temp. : 40deg-C