Effect of Sample Load on Peak Width (DEAE-825)

When IEC DEAE-825 (a column for weak anion exchange chromatography) is used, the peak does not widen until the sample load of ovalbumin reaches 1mg. When the quantity is increased to 4mg, the peak approximately doubles its width.

Sample : Ovalbumin

Column       : Shodex IEC DEAE-825 (8.0mmID*75mm)
Eluent       : (A); 20mM Piperazine-HCl buffer(pH6.0)
               (B); (A) + 0.5M NaCl
               Linear gradient: 0min to 20min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp .: Room temp.