When IEC SP-825 (a column for strong cation exchange chromatography) is used, the peak does not widen until the sample load of ribonuclease A reaches 1mg. When the quantity is increased to 5mg, the peak widths approximately 1.5 times.
Sample : Ribonuclease A
Column : Shodex IEC SP-825 (8.0mmID*75mm)
Eluent : (A); 20mM Sodium phosphate buffer(pH7.0)
(B); (A) + 0.5M NaCl
Linear gradient: 0 min to 20min, (A) to (B)
Detector : UV(280nm)
Column temp. : Room temp.