Feature of High Temperature Analysis (1) (High Resolution)

ET-RP1 4D is polymer base column for high temperature analysis. Figure shows comparison of reduced plate height and analysis time between 40deg-C and 150deg-C. Smaller value of reduced plate height means better column resolution. The reduced plate height of acetophenone and benzene decreased at the 150deg-C. Mobile phase viscosity decreases at higher temperature and thus enhanced diffusion rate between the eluent and the polymeric stationary phase. Then resolution is improved.

Sample :
1. Uracil
2. Pyridine
3. Acetophenone
4. Benzene
(notes) Eluent was introduced to column after preheating and introduced to detector after cooling.

Column : Shodex ET-RP1 4D (4.6mmID*150mm) : Shodex ET-RP1 4D (4.6mmID*150mm)
Eluent : H2O/CH3CN=50/50 : H2O/CH3CN=75/25
Flow rate : 0.5mL/min : 2.4mL/min
Detector : Diode array (210nm) : Diode array (210nm)
Column temp. : 40deg-C : 150deg-C

*Column oven : Polaratherm 9000 Series (SandraSelerity Technologies, Inc)

Coutesy of Research Institude for Chromatography bvba