Features of RSpak DE Series (2) (Adsorption of Metal Coordination Compound)

Since hinokititol and oxin-Cu are strong metal coordination compounds, they are adsorbed with the impurity metals in silica gel and the adsorption causes peak tailing. In case of RSpak DE series (polymer-based columns for reversed phase chromatography), the column is free from the effect of metals and sample will not be adsorbed by the column.

Sample : 5micro-L
1. 0.01% Hinokitiol
2. 0.001% Benzene
1. 0.01% Oxine-Cu

Column       : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent       : (Left); (1mM EDTA + 20mM KH2PO4) aq./CH3CN=60/40
               (Right); 20mM H3PO4 aq./CH3CN=95/5
Flow rate    : 1.0mL/min
Detector     : UV(254nm)
Column temp. : 40deg-C