AFpak AGE-894 (an affinity column) which has gelatin as the ligand was used to adsorb and elute fibronectin. Fibronectin is a glycoprotein which works to adhere and/or enlarge cell.

Sample : Fibronectin

Column       : Shodex AFpak AGE-894 (8.0mmID*50mm)
Eluent       : (A); 50mM Tris-HCl buffer(pH7.4) + 0.01M NaCl
               (B); (A) + 4M urea
               Step gradient: (A) to (B)
Flow rate    : 0.3mL/min
Detector     : UV(280nm)
Column temp. : Room temp.