Glutathione in Blood

With Asahipak GS-320 7G, a multimode column, it is possible to make the direct analysis of drug components in Serum and urine without sample pretreatment. Proteins, nucleic acids and polysaccharides are eluted first by GFC mode, and then, drugs and metabolites, whose hydrophobic interaction is larger, are eluted by reversed phase mode. This method can be used for the analysis of extracted fluid from serum, urine and internal organs.
GS-320 7G is an old type of GS-320 HQ.

Sample :
1. Protein
2. Glutathione

Column    : Shodex Asahipak GS-320 7G (7.5mmID*500mm)
Eluent    : 40mM EDTA-3Na(pH8.0)
Reagent   : 0.8mg/mL OPA in 20% CH3OH (for Post-column reaction)
Flow rate : 1.0mL/min 
Detector  : Fluorescence(Ex. 350nm, Em. 420nm)

Courtesy of Dr. Nimura, Kitazato Unversity