Histidine and 1-Methylhistidine

Histidine and 1-methylhistidine were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography)

Sample : 0.005% each, 5micro-L
1. Histidine
2. 1-Methylhistidine

Column       : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent       : 10mM Na2HPO4 aq.
Flow rate    : 0.5mL/min
Detector     : UV(210nm)
Column temp. : 30deg-C