Human control serum was analyzed using aqueous solvent SEC (GFC) high performance semi-micro columns, KW403-4F and KW404-4F. The bigger pore size (1,500A compared to 800A) of packed gel in KW404-4F is suitable for the analysis of large molecules that were excluded as void when using a KW403-4F column.
Sample : 1micro-L
Control serum I wako/B
Columns : Shodex KW403-4F, KW404-4F (4.6mmID*300mm each)
Eluent : 50mM Sodium phosphate buffer (pH7.0) + 0.3M NaCl
Flow rate : 0.20mL/min
Detector : UV(280nm)
Column temp. : 25deg-C