Human IgM (2)

IgM from human serum was analyzed using aqueous solvent SEC (GFC) columns, OHpak SB-805 HQ and SB-806 HQ.

Sample : 50micro-L
IgM, Immunoglobulin M from Human Serum
(Wako Pure Chemical Industries, Ltd.) 0.1%

Column     　: Shodex OHpak SB-806 HQ + SB-805 HQ (8.0mmID*300mm each)
Eluent       : 100mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate    : 1.0mL/min
Detector　   : UV(254nm) 
Column temp. : 30deg-C