Human Serum Albumin

Human serum albumin was analyzed using AFpak APD-894 (an affinity column).

Sample : Human serum albumin (Sigma)

Column       : Shodex AFpak APD-894 (8.0mmID*50mm)
Eluent       : (A); 0.02M Tris-HCl buffer(pH5.0)
               (B); (A) + 2.0M KCl
               Step gradient: (A) to (B) 
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : Room temp.