Lipoxidase (1) (SP-825)

Lipoxidase, an oxidoreductase was analyzed using IEC SP-825 (a column for strong anion exchange chromatography). It is a glycoprotein of MW 80,000. Lipoxidase from soybean consists of three fractions. Enzyme activity was found at two peaks around 25 minutes.

Sample: 0.5% Lipoxidase, 150micro-L

Column       : Shodex IEC SP-825 (8.0mmID*75mm)
Eluent       : (A); 20mM Citric acid-NaOH buffer(pH4.3)
               (B); (A) + 0.5M Na2SO4
               Linear gradient: 0min to 60min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : 25deg-C