Lipoxidase from Soybean (1) (QA-825, DEAE-825)

Lipoxidase from soybean was analyzed using IEC QA-825 (a column for strong anion exchange chromatography) and IEC DEAE-825 (a column for weak anion exchange chromatography). The enzyme activity of this sample was measured by using hydrogen peroxide as a substrate. Enzyme activity was found at the arrowhead position.

Sample : Lipoxidase from soybean

Columns      : Shodex IEC QA-825 (8.0mmID*75mm)           : Shodex IEC DEAE-825 (8.0mmID*75mm)
Eluent       : (A); 20mM Ethanolamine-HCl buffer(pH9.0)   : (A); 20mM Tris-HCl(pH8.0)
               (B); (A) + 0.5M NaCl                         (B); (A) + 0.5M NaCl
               Linear gradient:0min to 60min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : Room temp.