Nucleosides (5) (AIA-894)

The ligand of AFpak AIA-894 (an affinity column) is iminodiacetic acid. A custom column which is co-ordinated with cupper ion was used for the analysis of ribonucleosides. First, ribonuclosides were adsorbed, and then, released by 0.05M ethylmorpholine acetate(pH6.0). The sample was separated into four peaks.

Sample :
1. Uridine
2. Cytidine
3. Guanosine
4. Adenosine
5. 2′-Deoxyadenosine

Column       : Shodex AFpak AIA-894 (8.0mmID*50mm) (Cu co-ordinated using 0.05M cupric sulfate aq.)
Eluent       : 0.05M Ethylmorpholine acetate buffer(pH6.0)
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : Room temp.