Nucleosides (6) (APB-894)

AFpak APB-894 (an affinity column) which has aminophenyl boronic acid as the ligand was used to separate 2′-deoxyadenosine, uridine and adenosine.

Sample :
1. 2′-Deoxyadenosine
2. Uridine
3. Adenosine

Column       : Shodex AFpak APB-894 (8.0mmID*50mm)
Eluent       : 0.1M Sodium phosphate buffer(pH7.5)
Flow rate    : 0.5mL/min
Detector     : Shodex RI
Column temp. : 25deg-C