Nucleotides (10) (WA-624 pH5.5)
Nucleotides were analyzed using AXpak WA-624 (a column for weak anion exchange chromatography). pH of the eluent is heightened to 5.5.
Please compare with the cases of pH4.3 and pH5.0
Sample :
1. 5′CDP
2. 5′UDP
3. 5′ADP
4. 5′GDP
Column : Shodex AXpak WA-624 (6.0mmID*150mm) Eluent : 0.7M CH3COONH4 buffer(pH5.5) Flow rate : 1.0mL/min Detector : UV(260nm) Column temp. : 60deg-C