Nucleotides (3) (WA-624)

Nucleotides were analyzed using AXpak WA-624 (a column for weak anion exchange chromatography).

Sample : 20micro-L
1. 5′CMP 20μg/mL
2. 5′UMP 20μg/mL
3. 5′TMP 20μg/mL
4. 3′CMP 20μg/mL
5. 5′ADP 100μg/mL
6. 3′UMP 20μg/mL
7. 5′GMP 20μg/mL
8. 2′AMP 20μg/mL
9. 3′AMP 20μg/mL
10. 3′GMP 20μg/mL

Column       : Shodex AXpak WA-624 (6.0mmID*150mm)
Eluent       : 0.35M CH3COOH aq./0.35M CH3COONH4 aq.=240/100
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : 60deg-C