Nucleotides (4) (WA-624 pH5.0)

Ten nucleotides were separated using AXpak WA-624 (a column for weak anion exchange chromatography) with the eluent of pH5.0 isocratically. Compare with the cases of pH4.3 and pH5.5.

Sample :
1. 5′CMP
2. 5′UMP
3. 5′AMP
4. 5′GMP
5. 5′CDP
6. 3′AMP
7. 5′UDP
8. 5′ADP
9. 5′CTP
10. 5′UTP

Column       : Shodex AXpak WA-624 (6.0mmID*150mm)
Eluent       : 0.7M CH3COONH4 buffer(pH5.0)
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : 60deg-C