Nucleotides (5)

Nucleotides were analyzed using AXpak WA-624 (a column for weak anion exchange chromatography).

Sample :
1. 5′CMP , 2. 5′TMP
3. 5′IMP , 4. 5′AMP
5. 5′GMP , 6. 3′GMP
7. 3′CMP , 8. 5′UDP
9. 5′ADP , 10. 5′CTP
11. 5′UTP , 12. 5′ATP
13. 5′GTP

Column       : Shodex AXpak WA-624 (6.0mmID*150mm)
Eluent       : (A); 0.1M NaH2PO4 aq./CH3CN=90/10
               (B); 0.5M NaH2PO4 aq./CH3CN=80/20
               Linear gradient: 0min to30min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : 60deg-C