Nucleotides (7) (AAF-894)

Nucleotides were analyzed using AFpak AAF-894 (an affinity column). When (A) is used as the eluent, AMP isinulin was absorbed to the gel material. Then shortly after using (B) as the eluent, AMP elutes from the column.

Sample : Adenosine-5′-monophosphate, AMP

Column       : Shodex AFpak AAF-894 (8.0mmID*50mm)
Eluent       : (A); 0.01M 4-Ethylmorpholine acetate buffer(pH7.0)
               (B); 0.1M 4-Ethylmorpholine acetate buffer(pH7.0)
               Step gradient (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : Room temp.