Oligoadenylic Acids (4)

Oligoadenylic acids were analyzed using IEC DEAE-420N (a column for weak anion exchange chromatography packed with non-porous gel).
DEAE-420N is an old type of DEAE3N-4T.

Sample : Oligoadenylic acid, poly[A]n

Column       : Shodex IEC DEAE-420N (4.6mmID*35mm)
Eluent       : (A); 25mM Phosphate buffer(pH6.0)
               (B); (A) + 1M NaCl
               Linear gradient: 0min to 30min, 20% (B) to 100% (B)
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : Room temp.