Oligopeptides (SB401-4E)

Oligopeptides were analyzed using SB401-4E (a column for high perfonmance semi-micro aqueous SEC (GFC)).

Sample : 10micro-L
1. Gly-Gly-Gly
2. Gly-Gly
3. Glycine

Column       : Shodex SB401-4E (4.6mmID*250mm)
Eluent       : 50mM CH3COONH4 aq./CH3CN=90/10
Flow rate    : 0.2mL/min
Detector     : UV(210nm) (small cell volume) 
Column temp. : 25deg-C