Peptide Analysis (SEC)

Various peptides with MW ranged from 269 to 1734 was analyzed using aqueous solvent SEC (GFC) high performance semi-micro column, KW402.5-4F. Since each peptide may contain different amino-functional groups, the separation mode occur may not be as a “pure” size exclusion mode like for the analysis of proteins. This is more significant when peptides contain more hydrophobic-amino acids and/or basic-amino acids.

Sample : 5micro-L
1. Gly-Gly-His
2. Thrombin Receptor Activator Peptide (Ser-Phe-Leu-Leu-Arg-Asn-Pro-Asn-Asp-Lys-Tyr-Glu-Pro-Phe)
3. Angiotensin Ⅱ (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe)
4. Angiotensin Ⅰ (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu)
5. Leu-enkephalin (Tyr-Gly-Gly-Phe-Leu)

Column      : Shodex KW402.5-4F (4.6mmID*300mm)
Eluent       : 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate    : 0.33mL/min
Detector    : UV(220nm) 
Column temp. : 30deg-C