Poly-Lysine

Basic polypeptide Poly-Lysine was analyzed using PROTEIN KW-803 (a column for GFC separation).

Sample : 100micro-L
Poly-L-lysine hydrobromide (Sigma-Aldrich) 0.1%

Column     　: Shodex PROTEIN KW-803 (8.0mmID*300mm)
Eluent       : 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate    : 1.0mL/min
Detector　   : UV(220nm) 
Column temp. : 30deg-C