Using gradient elution, a sample containing proteins and peptides with a variety of molecular weight were separated. Asahipak ODP series provides good reproducibility of elution volume as well as high recovery for analysis of proteins and peptides.
Column : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent : 0.05% TFA aq./CH3CN
Linear gradient: 80/20 to 50/50, 20min
Flow rate : 1.0mL/min
Detector : UV(220nm)
Column temp. : 30deg-C