Proteins in Human Serum (1) (QA-825)

Proteins in Human serum were analyzed using IEC QA-825 ( a column for strong anion exchange chromatography ).
In this analysis, the sample was separated into four peaks including the transferrin peak; the largest one is serum albumin.

Sample : Human serum
1. IgG
2. Transferrin
3. (Unknown)
4. Serum albumin

Column       : Shodex IEC QA-825 (8.0mmID*75mm)
Eluent       : (A); 20mM Tris-HCl buffer(pH8.6)
               (B); (A) + 0.5M NaCl
               Linear gradient: 0min to 60min, 100% (A) to 50% (B)
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : Room temp.