Purine Bases, Pyrimidine Bases and Nucleosides (1) (DE-613)

Purine bases, pyrimidine bases and nucleosides were analyzed using RSpak DE-613 (a column for reversed phase chromatography).

Sample :
1. Cytosine
2. Cytidine
3. Uracil
4. Hypoxanthine
5. Uridine
6. Inosine
7. Guanine
8. Tymine
9. Guanosine
10. Adenine
11. Thymidine
12. Adenosine

Column       : Shodex RSpak DE-613 (6.0mmID*150mm)
Eluent       : (A); 0.05M KH2PO4 aq.
               (B); 50% CH3CN
Flow rate    : 1.0mL/min
Detector     : UV(254nm)
Column temp. : 22deg-C