Purine Bases, Pyrimidine Bases and Nucleosides (2) (NN-814)

Six components were separated using RSpak NN-814 with pH3.0 phosphate buffer as the eluent. Since cation exchange mode is contributing to the separation, the elution order is different from that of the separation with RSpak DE-613.

Sample : 20micro-L
1. Uridine 50micro-g/mL
2. Uracil 50micro-g/mL
3. Guanosine 100micro-g/mL
4. Cytidine 100micro-g/mL
5. Adenosine 50micro-g/mL
6. Cytosine 50micro-g/mL

Column       : Shodex RSpak NN-814 (8.0mmID*250mm)
Eluent       : 100mM NaH2PO4 aq./100mM H3PO4 aq.=500/66
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : 25deg-C