Recovery of Proteins and Enzime Activity (QA-825)

Recoveries of proteins and enzime activity for IEC QA-825 (a column forstrong anion exchange chromatography) are shown. When 0.5mg of each proteins was injected into the column by linear gradient elution, in which salt concentration increases linearly over one minute, sample recovery was found to be good. Then the same amount of enzyme was injected into the column in the same manner as the above protein and its recovery was also found to be good.

Recovery of proteins
Protein Recovery (%)
Ovalbumin 106
Conalbumin 100
beta- Lactoglobulin 99
Trypsin inhibitor 99
Recovery of enzyme activity
Enzyme Recovery (%)
Kallikrein 95
Lipoxidase 100
Catalase 96

Sample load : 0.5mg each

Column : Shodex IEC QA-825 (8.0mmID*75mm)
Eluent : (A); 20mM Tris-HCl buffer(pH7.5)
         (B); (A) + 0.5M NaCl
         Linear gradient: (A) to (B), 1min