Recovery of Proteins and Enzyme Activity (DEAE-825)
The tables show good recovery of IEC DEAE-825 (a column for ion exchange chromatography) for proteins and enzyme activity.
| Recovery of proteins | |
|---|---|
| Protein | Recovery (%) |
| Trypsin inhibitor | 96 |
| beta-Lactoglobulin | 100 |
| Ovalbumin | 101 |
| Myoglobin | 100 |
| gamma-Globulin | 99 |
| BSA | 97 |
| Thyroglobulin | 90 |
| Recovery of enzyme activity | |
|---|---|
| Enzyme | Recovery (%) |
| Kallikrein | 92 |
| Lipoxidase | 96 |
| Catalase | 94 |
Sample load : 0.5mg each
Column : Shodex IEC DEAE-825 (8.0mmID*75mm)
Eluent : (A); 20mM Tris-HCl buffer(pH8.0)
(B); (A) + 0.5M NaCl
Linear gradient: 0min to 1min, (A) to (B)